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KMID : 0895820140240020041
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 2 p.41 ~ p.50
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Protective Effect of Clematidis Radix Extract on CoCl2-induced Apoptosis in Human Neuroblastoma Cells
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Park Jung-Woo
Lim Hyung-Ho
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Abstract
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Objectives : The purpose of this study was to evaluate the effects of Clematidis radix ex-tract on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.
Methods : In order to investigate the protective effect of Clematidis radix on CoCl2-inducedcytotoxicity in neuronal cells, MTT(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bro-mide) assay, DAPI(4,6-diamidino-2-phenylindoleI) staining, TUNEL (terminal deoxynucleo-tidyl transferase-mediated dUTP nick end-labeling) assay, DNA fragmentation assay and western blotting were performed on SH-SY5Y human neuroblastoma cells.
Results : Cells treated with CoCl2 exhibited several apoptotic features, while cells pre-treat-ed with Clematidis radix prior to CoCl2 exposure showed a decrease in the occurrence of apoptotic features. CoCl2 increased HIF-1α expression, in contrast, Clematidis radix treat-ment decreased CoCl2-induced HIF-1α expression. Pre-treatment with the extract of Clematidis radix suppressed Bax, cytochrome c, and caspase-3 expressions, and also in-creased Bcl-2 expression in SH-SY5Y human neuroblastoma cells.
Conclusions : These results suggest that Clematidis radix may exert a protective effect on CoCl2-induced apoptosis in SH-SY5Y human neuroblastoma cells.
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KEYWORD
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Clematidis radix, CoCl2, Hypoxia, Apoptosis, SH-SY5Y human neuro-blastoma cells
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